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In the drought tolerance assay, 12-day-old seedlings that had been cultivated on MS medium were transferred to soil that was saturated with water, and then subjected to drought treatment by withholding water while being grown under long-day conditions (16 h light/8 h darkness) at 22 C

If symptoms are persistent or significantly restrict dietary intake, referral to a registered dietitian should be considered to ensure nutritional needs continue to be met

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